FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n02_NON180-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n02_NON180-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169501799
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG190100.025.0384775
GTCTAGG467550.021.3776441
TATACCG490550.020.5874355
GTATCAT5072800.018.7533494-95
CCTAGAC484200.018.3458773
CGTATCA6207150.018.24564294-95
ATCATTA3600150.018.14515594-95
CCGTATC7541600.017.90879894-95
TATCATT4434950.017.81214394-95
TAGAGCG426450.017.633365
GTACCGT291150.017.3109596
GGACTGC384150.017.0895146
ACTGTCC284850.016.7585988
AGTACCG406450.016.7479385
TCATTAA2969600.016.65066194-95
GCCGTAT9895650.016.55213594-95
GTCTATG543550.016.4483471
GTATAAG808150.016.423911
CGCCGTA12075600.016.31640294-95
CACTGAG599800.016.2422717
Produced by FastQC (version 0.11.5)