FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n02_NON180-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n02_NON180-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93455885
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA3437100.024.46396494-95
ATCATTA4626950.023.97627694-95
CATTAAA2382500.022.93174694-95
TAGACGG85100.022.663035
TATCATT6000400.022.00781294-95
GTCTAGG222800.020.1064411
GTATCAT7373900.019.68449894-95
GTACCGT137750.019.6219626
TATACCG221000.019.603075
ACTGTCC130650.018.4326788
AGTACCG197250.018.4232375
TACCGTG173150.017.748917
TAGAGCG222750.017.7430045
CCTAGAC237450.017.7048453
CTAGACA235400.017.2940084
GGACTGC170450.017.2789136
CGTATCA9035100.017.11307394-95
AAGACCG198450.016.8038025
GTATAAG371550.016.5829351
ACCGTGA215500.016.4209738
Produced by FastQC (version 0.11.5)