FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n02_NON15-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n02_NON15-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123743811
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA4960300.024.82273394-95
ATCATTA6640450.024.19996594-95
CATTAAA3440800.023.42026794-95
TATCATT8579150.022.13306894-95
GTCTAGG308050.022.006411
TAGACGG104300.021.8617615
GTACCGT163400.021.6005946
TATACCG267500.021.523215
CTAGACA285450.020.3028894
GTATCAT10681850.020.02876394-95
AGTACCG241350.019.7809435
CCTAGAC317550.019.5819023
TACCGTG207250.019.572727
TAGAGCG272200.019.4238475
CTAGACG120600.019.2220884
ACTGTCC156600.019.1089278
GGACTGC201100.018.6613886
CACTGAG365650.017.9137527
ACCGTGA251050.017.7850518
GACTGCG94600.017.6239747
Produced by FastQC (version 0.11.5)