FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n02_NON15-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n02_NON15-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108059119
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA3433850.023.13224894-95
ATCATTA4479000.022.47125494-95
CATTAAA2449800.021.19275794-95
TATCATT5652050.020.41317694-95
TAGACGG109450.020.138625
TATACCG271150.019.2187215
GTATCAT6825200.018.78632294-95
GTCTAGG263000.018.0810361
GTACCGT177650.017.5147446
AGTACCG243950.016.7660145
CGTATCA8224650.016.65619394-95
CCTAGAC275300.016.4959893
ACTGTCC166050.016.4195218
TAGAGCG246750.016.2099785
GTATAAG455550.015.6944261
GGACTGC221650.015.4523746
TACCGTG226650.015.2777677
CTAGACA313300.015.1472074
TATACTG451550.015.0543255
CCGTATC9740150.015.04849494-95
Produced by FastQC (version 0.11.5)