FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n02_EXW60-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n02_EXW60-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90724727
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1509880.1664243089979207No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG96450.022.8528885
TCATTAA2083500.021.90703694-95
ATCATTA2710050.021.75083294-95
GTACCGT174950.021.2605066
TATACCG244100.020.8229065
AGTACCG235850.020.2018175
GTCTAGG257850.020.008041
TATCATT3380000.019.8202594-95
CATTAAA1481800.019.52851394-95
GTATCAT3988650.018.38620694-95
GTCTATG290900.018.159461
TAGAGCG256300.018.0524315
CCTAGAC251300.017.84453
GTATAAG379000.017.6734681
CTAGACA278400.017.6602254
ACTGTCC173100.017.0405648
TACCGTG205300.016.866627
CGTATCA4782850.016.44447594-95
TACACCG196100.016.3754945
GGACTGC207850.016.2725586
Produced by FastQC (version 0.11.5)