FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n02_EXW60-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n02_EXW60-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114623231
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1768380.154277626321666No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA3020050.022.72922594-95
ATCATTA4004550.022.44412894-95
TATCATT5063200.020.65682494-95
CATTAAA2150500.020.56527394-95
GTACCGT205950.020.5282296
TATACCG302750.020.1466925
TAGACGG121050.019.739045
GTCTAGG304800.019.3262841
GTATCAT6130300.019.01759794-95
AGTACCG280200.018.4282595
TAGAGCG305750.017.2611795
CGTATCA7416500.016.96745394-95
CCTAGAC316700.016.619353
TACCGTG253450.016.0424067
GTCTATG350250.015.9368081
GTATAAG430950.015.8626421
CTAGACA333750.015.8415184
CACTGAG471850.015.7844747
ACTGTCC205150.015.6749148
CCGTATC8851300.015.51894294-95
Produced by FastQC (version 0.11.5)