FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n02_EXW5D-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n02_EXW5D-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75103777
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1804730.24029816769401627No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC2017750.016.6437394-95
CGTATCA1693050.015.77740394-95
GCCGTAT2720600.015.2107994-95
CGCCGTA3278250.014.28095494-95
GTATCAT1478250.013.99390494-95
TATACCG231450.013.2587335
TAGAGCG256450.013.2443295
TCATTAA1090800.013.08355194-95
GTCTAGG240750.013.0430591
TAGACGG112950.012.4489345
CATTAAA962650.012.40002894-95
GTACCGT187600.012.0531556
CCTAGAC225200.011.9602713
AGTACCG227550.011.7115045
ATCATTA1218100.011.50760494-95
TATACTG326700.011.2106775
TATCATT1401700.011.18636794-95
GTATAAG295200.011.0878461
TCGCCGT4019250.010.95139194-95
GTGTATG340800.010.7751721
Produced by FastQC (version 0.11.5)