FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n02_EXW5D-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n02_EXW5D-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98726272
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATTA2605000.019.2126694-95
TCATTAA2086650.018.88845694-95
GTATCAT3776500.018.28454494-95
TATCATT3226950.018.28292594-95
TATACCG272050.017.4439245
CGTATCA4534400.017.35388494-95
CATTAAA1610050.016.53617594-95
CCGTATC5473650.016.44966594-95
TAGACGG124050.016.1600655
GCCGTAT6820550.014.98967994-95
GTACCGT178950.014.7594626
GTCTAGG278800.014.6878631
TAGAGCG245300.014.2724115
CGCCGTA8097700.014.00433194-95
AGTACCG233050.014.003455
GTATAAG408900.013.4418921
TATACTG442700.013.4345235
CCTAGAC281350.013.3216013
GTATAGG365250.013.0973491
GTGTATG389500.012.7453861
Produced by FastQC (version 0.11.5)