FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n02_EXW180-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n02_EXW180-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114455260
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1439070.12573209828888599No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG126700.025.2328265
TATACCG327600.021.9682875
GTCTAGG334550.021.6690251
GTACCGT213200.020.699346
TAGAGCG316550.019.9288945
AGTACCG296950.019.6445675
GTATCAT2940750.018.92755394-95
GTCTATG379550.018.9246851
CTAGACA363500.018.6616944
CGTATCA3589900.018.59124694-95
CCTAGAC337900.018.4587753
CCGTATC4391700.018.37204694-95
TACCGTG264450.017.9257137
TATCATT2557800.017.75190594-95
GGACTGC257650.017.7182676
ATCATTA2111450.017.6812694-95
GTGTATG432550.017.3526821
CACTGAG497800.017.0990757
ACTGAGC455000.017.089398
ACTGTCC210200.016.9705738
Produced by FastQC (version 0.11.5)