FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n02_EXW180-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n02_EXW180-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111130318
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1507820.1356803460240256No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG114050.028.1980155
GTCTAGG331850.023.2767771
GTACCGT204500.022.5096
TATACCG339750.021.5321035
AGTACCG286800.020.6543925
CCTAGAC331800.019.9291063
CTAGACA359200.019.8239634
TAGAGCG311500.019.5961275
ACTGTCC188700.019.4579288
TACCGTG254700.019.2832667
GTCTATG377800.018.9745461
GTATCAT2735500.018.9472694-95
GGACTGC242650.018.461086
CGTATCA3314600.018.46009894-95
CCGTATC3993500.018.08196894-95
TATCATT2379950.017.91985994-95
GTGTATG434750.017.8548641
GCCCTAG203450.017.8160291
ATCATTA1972600.017.7230194-95
TACACCG220800.017.5771375
Produced by FastQC (version 0.11.5)