FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n02_EXW15-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n02_EXW15-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88974071
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1310620.1473035891546426No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG100650.021.0024915
TCATTAA1712250.019.8699294-95
ATCATTA2127450.019.81673694-95
GTCTAGG264500.019.0920311
TATACCG258950.018.9683345
GTACCGT180600.018.043926
TATCATT2589400.018.00119494-95
CATTAAA1260700.017.45806594-95
GTATCAT2943850.017.37957294-95
AGTACCG244050.016.9926055
CCTAGAC249250.016.466563
GTCTATG303150.016.172111
TAGAGCG267700.015.9705075
CGTATCA3463400.015.78734494-95
GGACTGC210900.015.5416486
GTATAAG391600.015.3337721
ACTGTCC175700.015.3014348
GTGTATG342500.015.1879141
CTAGACA296900.015.1518034
CCGTATC4044000.014.95903494-95
Produced by FastQC (version 0.11.5)