FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n02_EXW15-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n02_EXW15-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125853497
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1791180.1423226245354152No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG143150.023.9923635
TATACCG387850.020.5765025
GTACCGT220400.019.9368276
ATCATTA3068300.019.34509594-95
GTCTAGG365450.019.3428081
TCATTAA2454400.019.19545294-95
AGTACCG313900.018.3718745
TATCATT3693250.017.99634694-95
GTATCAT4246850.017.73080894-95
TAGAGCG349350.017.6906035
CATTAAA1813350.017.07380994-95
GTCTATG403850.016.9624961
TACCGTG277150.016.9157227
CGTATCA5077150.016.6363694-95
CTAGACA414600.016.556354
GGACTGC279350.016.5288816
CCTAGAC362700.016.515553
CCGTATC6028900.016.15349494-95
GTATAAG580450.016.0002381
TATACTG538400.015.7933435
Produced by FastQC (version 0.11.5)