FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85225265
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT58800.019.3098056
AGTACCG83350.017.555275
TAGACGG44100.017.236295
GTCTATG114600.017.1040941
ACTGTCC65650.015.4049868
TACCGTG76950.015.2409477
GTATAGG132250.015.2171081
GTGTATG124500.015.0179491
GCCCTAG69050.014.7447541
ACCGTGA106300.014.4719898
GTCCTGG72850.014.4327861
GTCTAGT78800.014.0675081
CACTGAG136250.014.0440427
TAAGGTG110900.013.9224025
TAGAGGT110450.013.721054
GTCTTAG89000.013.631311
TATACTG160550.013.5820325
GTCTAGA118000.013.3857761
GCATAGG91500.013.0508891
GTCGGTC129550.013.0475528
Produced by FastQC (version 0.11.5)