FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n01_SAL60-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n01_SAL60-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71189877
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG83550.028.027685
GTACCGT118600.021.4265886
GTCTAGG214400.021.32291
TATACCG245400.021.0010915
AGTACCG171300.020.269675
GGACTGC161750.019.586896
CTTCTGC1171700.018.97199694-95
GACGGTC105650.018.7476757
CCTAGAC217450.018.6332453
TAGAGCG189550.018.117625
ACTGTCC123850.018.025318
ACTGAGC253800.017.6669378
GCCGTCT3212900.017.64488494-95
GTCTTCT1812450.017.60513394-95
TCTTCTG1554300.017.57063594-95
GCCCTAG129100.017.4112871
TACCGTG151750.017.3718137
CCGTCTT2625600.017.36348794-95
CGTCTTC2160250.017.32265394-95
CACTGAG287550.017.3112647
Produced by FastQC (version 0.11.5)