FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n01_SAL5D-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n01_SAL5D-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97775738
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG92600.027.442765
TTCTGCT2722350.024.31149594-95
CTTCTGC3653600.022.9658494-95
TATACCG282250.022.331715
GTCTAGG266950.022.1810871
TCTGCTT1969700.021.84051594-95
GTACCGT146050.021.5623056
TACCGTG185500.020.7662337
AGTACCG208000.020.4382975
CCTAGAC287700.020.1425233
TCTTCTG4834400.019.76336394-95
CTAGACA297450.019.3381634
GGACTGC206750.019.2752346
GTGTATG352250.019.0762541
GTATAAG469850.018.9744111
ACTGAGC279450.018.7308838
TACACCG200750.018.4554255
CACTGAG313600.018.2512177
CTGCTTG1251950.017.91511394-95
TAGAGCG224950.017.7791565
Produced by FastQC (version 0.11.5)