FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n01_SAL5D-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n01_SAL5D-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50701749
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT2320900.015.54102794-95
TCTGCTT921600.015.20039994-95
CCGTCTT2140350.014.97104494-95
GCTTGAA463700.014.7160594-95
CTGCTTG705950.014.71289894-95
TGCCGTC3006850.014.64899594-95
TCCGTCT575500.014.16841394-95
TGCTTGA556850.014.00740494-95
TTCCGTC715950.013.89034494-95
TTCTGCT986700.013.54276594-95
GTATAGG240050.012.9665051
TATACCG142650.012.5531675
ATTCCGT764900.012.50770294-95
ATGCCGT3177150.012.41348994-95
CGTCTTC1892350.012.2890594-95
CTTCTGC1169050.012.0939392-93
TATGCCG3499100.012.06745392-93
TATACTG222550.011.9734425
CCTAGAC135500.011.9539453
TATTCCG816600.011.93028892-93
Produced by FastQC (version 0.11.5)