FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n01_SAL180-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n01_SAL180-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42964952
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT1132650.016.9795994-95
TGCCGTC1430950.016.48749594-95
CCGTCTT903050.015.40501794-95
ATGCCGT1595700.014.76140994-95
TAGACGG62900.013.6682435
GTCTAGG142600.013.4630291
TATGCCG1780000.013.24010592-93
CCTAGAC137950.012.8434443
TATACCG141050.012.7629455
CGTCTTC824600.012.72856494-95
GTATGCC1971300.011.95284292-93
TAAGGTG219550.011.8774635
TAGAGCG145900.011.785235
TAGAGGT188250.011.5057424
CGTATGC2054250.011.46537890-91
CTAGACA201500.011.432774
CCAGGAC132800.011.3384993
CTAAGGT177050.011.2141194
GTCTAGA150600.011.0439391
GTATAAG168500.010.9706061
Produced by FastQC (version 0.11.5)