FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n01_SAL180-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n01_SAL180-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82547904
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT3056050.025.37481194-95
CTTCTGC4177000.023.61743294-95
TCTGCTT2147450.023.1604594-95
TAGACGG83100.021.7203795
TATACCG216650.020.5211645
TCTTCTG5531200.020.42411694-95
GTACCGT120400.020.1988776
GTCTAGG207700.019.5161511
AGTACCG168250.019.1125415
CTGCTTG1350350.019.0421194-95
GGACTGC162550.018.6722936
GTCTTCT6642450.018.01704694-95
GTATAAG353350.017.7521291
ACTGTCC128900.017.3191418
TACCGTG152900.016.6820057
CTAGACA241150.016.2893264
CCTAGAC228200.016.256633
GTCTATG238500.016.0792881
GTGTATG280800.016.0263461
ACTGAGC233150.015.8498678
Produced by FastQC (version 0.11.5)