FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n01_SAL15-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n01_SAL15-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67980617
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG62850.023.7307115
TTCTGCT1757950.023.49140794-95
CTTCTGC2341700.022.82342394-95
GTACCGT99000.021.9742036
TATACCG190500.021.8172235
GTCTAGG182100.021.6856941
TCTGCTT1280800.020.235594-95
AGTACCG145200.019.9549035
TCTTCTG3131000.019.7734494-95
TACCGTG126550.019.2547027
GGACTGC132200.018.9707876
GTGTATG243650.018.4699541
CCTAGAC197200.018.450953
ACTGTCC95950.018.415338
GTCTTCT3751500.018.10406194-95
ACTGAGC193850.017.568518
TAGAGCG152050.017.4939545
GACTGTG72400.017.4511767
GTATAAG316800.017.4302351
TATACTG299900.017.2638025
Produced by FastQC (version 0.11.5)