FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n01_NON60-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n01_NON60-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101737024
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG103750.028.2017975
TTCTGCT2898400.024.17963894-95
CTTCTGC3926850.022.76201894-95
GTCTAGG272600.021.826361
TCTGCTT2137500.021.23944794-95
GTACCGT172050.020.2915296
TATACCG303900.019.959265
TCTTCTG5193650.019.9201794-95
ACTGTCC165100.019.850988
CCTAGAC289850.019.4195753
AGTACCG246350.018.3556025
GTCTTCT6214300.017.9166294-95
GTCTATG321050.017.896021
GACGGTC137750.017.7235817
TACCGTG224100.017.6556247
CTGCTTG1339650.017.579294-95
CTAGACA315400.017.514684
GGACTGC211800.017.4700286
GTGTATG365200.017.4632321
ACTGAGC350400.017.3374988
Produced by FastQC (version 0.11.5)