FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n01_NON60-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n01_NON60-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108287194
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG96300.025.796515
TTCTGCT3699000.025.46506394-95
CTTCTGC5149400.024.17117794-95
TCTGCTT2622650.022.85213794-95
GTCTAGG259300.021.9929121
TATACCG273200.021.5588995
GTACCGT165800.021.428896
TCTTCTG7000150.021.32331894-95
ACTGTCC153550.020.323378
GTCTTCT8551800.019.08833994-95
CTGCTTG1611850.019.02833694-95
CCTAGAC287650.018.9242233
AGTACCG240300.018.916635
TAGAGCG259500.018.688485
GGACTGC194600.018.0134186
TACCGTG217800.017.81747
ACTGAGC342100.017.7026088
AAGACCG242300.017.4862635
CACTGAG365600.017.2922697
GTATAAG449150.017.235891
Produced by FastQC (version 0.11.5)