FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n01_NON5D-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n01_NON5D-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55276869
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG81300.015.1904545
GCCGTCT645850.014.19878194-95
TATACCG196000.013.8862995
CCGTCTT679100.013.67145994-95
GTCTAGG162300.013.6428751
TGCCGTC994500.013.340713594-95
CCTAGAC160800.013.2338153
GTACCGT127500.011.9959126
ATGCCGT876600.011.66690194-95
TATACTG247450.011.6516975
AGTACCG169900.011.3506475
ACTGTCC127850.011.2942988
TAGAGCG171900.011.2185865
GTATAAG261150.011.1716371
GTCCTGG162300.011.1250911
GTGTATG264950.011.04727651
GGACTGC155150.011.0214396
CGTCTTC653600.010.99249694-95
CTAGACT169850.010.9345054
ACTGAGC260400.010.9080378
Produced by FastQC (version 0.11.5)