FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n01_NON5D-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n01_NON5D-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62999288
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT2719650.014.92198994-95
TGCCGTC3317050.014.54095994-95
TCTGCTT1139200.014.15067794-95
CCGTCTT2206700.013.64281694-95
CTGCTTG911050.013.59340294-95
GCTTGAA595300.013.3462694-95
TGCTTGA772550.013.223371594-95
TTCTGCT1291900.013.02597794-95
ATGCCGT3669700.012.94101894-95
TATGCCG4022600.011.95709892-93
CTTCTGC1422850.011.39889492-93
CGTCTTC2004650.011.37567794-95
GTCTAGG199200.011.2362061
TATACCG172150.011.1470535
GTATGCC4362800.010.89515292-93
TAGAGCG152450.010.8738595
TCTTCTG1590400.010.63854592-93
CGTATGC4512600.010.62967390-91
CCTAGAC189900.010.40551953
TATACTG304500.010.3265775
Produced by FastQC (version 0.11.5)