FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n01_NON180-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n01_NON180-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169501799
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG190200.026.6464215
GTCTAGG466700.022.7885551
TATACCG501350.020.5590825
GTACCGT297050.018.9803686
TAGAGCG435950.018.9473275
CCTAGAC497450.018.7441713
CTTCTGC2770200.018.46340494-95
ACTGTCC285850.018.3281218
GGACTGC378400.018.2890826
AGTACCG412400.018.1173655
GTCTTCT4242150.017.89951594-95
CCGTCTT6739700.017.59656594-95
CGTCTTC5578000.017.5401294-95
ACTGAGC591750.017.4582888
TACACCG363550.017.4291695
GTGTATG638050.017.3463971
TCTTCTG3652300.017.24146894-95
GCCGTCT7696900.017.14620694-95
GACGGTC261550.017.1071177
CACTGAG621900.017.0701627
Produced by FastQC (version 0.11.5)