FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n01_NON180-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n01_NON180-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93455885
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT3071100.025.50270894-95
TAGACGG88900.024.4709285
CTTCTGC4368850.024.25707494-95
TCTGCTT2185200.023.0430694-95
TCTTCTG5955400.021.66707294-95
GTACCGT132700.021.4049856
GTCTAGG226600.020.7615091
ACTGTCC131500.020.6249188
TATACCG230400.020.2036765
GTCTTCT7273450.019.3440894-95
CTGCTTG1344500.019.1126994-95
AGTACCG198050.018.8989725
TAGAGCG223100.018.416345
GGACTGC177100.018.3451926
CCTAGAC237750.018.1809843
TACCGTG176750.017.8439337
CTAGACA245150.017.5155324
GTATAAG376900.017.4373171
GACTGTG96900.016.9603277
GACAGTG133600.016.9231957
Produced by FastQC (version 0.11.5)