FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n01_NON15-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n01_NON15-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123743811
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG97950.027.3016415
TTCTGCT4897250.026.04562494-95
CTTCTGC6825950.024.54324594-95
TCTGCTT3407450.024.1836394-95
GTACCGT166400.023.835026
GTCTAGG293700.022.0866641
TATACCG267600.021.8857675
TCTTCTG9378450.021.66557994-95
ACTGTCC156150.021.657968
CTAGACA291500.021.5578924
CTGCTTG2062250.020.66225694-95
TACCGTG213900.020.3850357
CCTAGAC330950.020.3808463
AGTACCG246000.019.984355
GTCTTCT11540350.019.63601394-95
ACCGTGA266850.019.1168638
GGACTGC205750.018.930256
TAGAGCG269350.018.8162165
AAGACCG233300.018.730875
ACTGAGC340100.018.5887838
Produced by FastQC (version 0.11.5)