FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n01_NON15-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n01_NON15-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108059119
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT3338850.024.25090294-95
CTTCTGC4493600.022.96059894-95
TCTGCTT2404950.021.7297194-95
TAGACGG111500.020.6609575
GTCTAGG260250.020.1958371
TCTTCTG6019600.020.15255294-95
GTACCGT176400.019.144916
TATACCG267600.018.5487125
GTCTTCT7263850.018.37014894-95
CTGCTTG1502050.018.01413394-95
CCTAGAC278950.017.9306343
AGTACCG245350.017.152655
GGACTGC225400.016.6898986
TAGAGCG256750.016.6870545
TACCGTG224200.016.6096637
ACTGTCC170450.016.4134038
TATACTG457850.016.2358385
CGTCTTC8763500.016.18994-95
GTATAAG462250.016.1200581
ACTGAGC329250.015.9843278
Produced by FastQC (version 0.11.5)