FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n01_EXW60-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n01_EXW60-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90724727
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG100600.026.0159115
TTCTGCT1976050.023.18165494-95
GTCTAGG252350.022.5033441
TATACCG263750.022.4934145
CTTCTGC2733350.021.90847894-95
GTACCGT179550.021.7455146
GTCTATG292450.020.0839581
AGTACCG251900.019.9688575
TCTTCTG3503350.019.85870294-95
TCTGCTT1485750.019.47160594-95
CCTAGAC262050.018.7788983
ACTGTCC171550.018.7724118
TAGAGCG274900.018.0907825
GTCTTCT4215700.017.94485394-95
GGACTGC199050.017.8970876
TACCGTG214900.017.7042377
CTAGACA280900.017.6705464
TATACTG359000.017.6633155
GTGTATG319500.017.654781
GTATAAG372550.017.4367851
Produced by FastQC (version 0.11.5)