FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n01_EXW60-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n01_EXW60-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114623231
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT2928650.023.37165694-95
TAGACGG127600.022.5956215
CTTCTGC4049750.022.28742894-95
TATACCG309250.021.103935
TCTTCTG5283450.020.41529894-95
GTACCGT209250.020.3842126
TCTGCTT2160150.020.1368994-95
GTCTAGG316250.019.2642381
AGTACCG287000.018.9500275
GTCTTCT6418550.018.61962794-95
TAGAGCG308300.018.1800375
TACCGTG258550.017.4158767
CCTAGAC309800.017.141843
ACTGTCC211700.016.6929728
GTCTATG342600.016.5064621
CGTCTTC7857900.016.45495894-95
ACTGAGC449050.016.342388
GTATAAG443150.016.1927471
GGACTGC254300.016.0633146
CTGCTTG1430100.015.92077394-95
Produced by FastQC (version 0.11.5)