FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n01_EXW5D-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n01_EXW5D-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75103777
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT2202800.015.75867594-95
GCCGTCT2723950.015.69267294-95
TAGACGG111600.014.6413895
TGCCGTC3297200.014.38058294-95
GTCTAGG229900.014.1178081
CGTCTTC1991550.013.98236194-95
TATACCG236050.013.5425075
TTCTGCT1074850.013.29621694-95
TAGAGCG256500.013.1664975
GTCTTCT1632600.013.12413994-95
ATGCCGT3573350.012.38786294-95
TATGCCG3910250.012.09540992-93
GTACCGT193900.011.9299186
TCTGCTT999700.011.91983794-95
CCTAGAC238150.011.7878553
TATACTG327100.011.7768285
GTATAAG295500.011.4822311
GTCTATG274150.011.44041
TCTTCTG1435050.011.35741794-95
CTTCTGC1286350.011.08421894-95
Produced by FastQC (version 0.11.5)