FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n01_EXW5D-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n01_EXW5D-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98726272
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT2012700.019.78319594-95
CTTCTGC2610750.019.48094794-95
TCTTCTG3385150.018.2583194-95
GTCTTCT4032600.017.80300794-95
TATACCG273050.017.3958035
TCTGCTT1585050.016.87303594-95
CGTCTTC4952450.016.51976694-95
CCGTCTT5801550.016.05484294-95
GTACCGT182600.015.8936656
TAGACGG119150.015.7467075
GTCTAGG278250.015.2681561
AGTACCG231550.015.2210915
GCCGTCT6958450.015.16771894-95
GTATAAG401300.014.8495281
CCTAGAC283350.014.5175253
ACTGTCC193750.014.12089358
ACTGAGC344450.014.0103478
TGCCGTC8278850.013.94439994-95
TATACTG437000.013.8041285
CTGCTTG1104800.013.69043494-95
Produced by FastQC (version 0.11.5)