FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n01_EXW180-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n01_EXW180-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114455260
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG118900.025.926725
GTACCGT223200.023.3450766
GTCTAGG341550.023.096891
TATACCG337350.022.8096735
AGTACCG304250.021.5131195
TAGAGCG325000.020.9434175
CCTAGAC341350.020.288673
GTCTATG376950.020.2218271
TACCGTG272750.019.1387047
CACTGAG522550.019.1339177
GGACTGC252150.019.02596
ACTGAGC474500.018.8692558
CTTCTGC2069750.018.62156994-95
GTGTATG431650.018.5730441
ACTGTCC204400.018.4742098
GTCTTCT3192550.018.44313494-95
TCTTCTG2659200.018.17824794-95
TTCTGCT1564400.018.14480894-95
TACACCG246900.018.0838765
GTATAAG522750.018.0453871
Produced by FastQC (version 0.11.5)