FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n01_EXW180-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n01_EXW180-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111130318
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG122950.027.429325
GTCTAGG326900.024.0734671
GTACCGT198250.022.9767136
TATACCG342000.022.7912275
GTCTATG371750.020.8878751
AGTACCG284250.020.8544795
TAGAGCG309650.020.4016725
CCTAGAC340800.019.610593
ACTGTCC195300.019.5782648
TACCGTG253600.019.5350977
CACTGAG485500.019.1168297
GGACTGC245250.018.9414126
CTTCTGC1886300.018.89887294-95
GTGTATG436400.018.8715251
CTAGACA372800.018.8569324
GTATAAG522950.018.5743641
GTCTTCT2906300.018.47800394-95
TCTTCTG2428050.018.41694-95
ACTGAGC443700.018.3592628
CGTCTTC3527400.017.90502294-95