FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n01_EXW15-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n01_EXW15-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88974071
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG101100.022.504515
GTCTAGG265450.019.4956571
TATACCG264650.019.2401355
GTACCGT183600.018.2648686
AGTACCG242650.017.4023025
GTCTATG304050.017.1300351
CCTAGAC267900.017.0213013
GTGTATG343100.016.7039551
CACTGAG431650.016.0658287
TAGAGCG269050.015.9948595
GTATAAG379200.015.7278041
GGACTGC213800.015.6404576
ACTGTCC183250.015.578038
TATACTG370200.015.5635845
ACTGAGC400100.015.5400778
CTAGACA308300.015.4992234
CTTCTGC1128600.015.25356794-95
TCTGCGT214800.015.02721594-95
TCTTCTG1279700.014.93362194-95
GTCTTCT1391050.014.93173794-95
Produced by FastQC (version 0.11.5)