FastQCFastQC Report
Fri 2 Oct 2020
HVF5LDRXX_n01_EXW15-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5LDRXX_n01_EXW15-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125853497
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT224350.021.2987886
TTCTGCT2278850.021.15620894-95
TATACCG381900.021.1190285
GTCTAGG364300.020.4417061
CTTCTGC3024650.020.24690494-95
TAGACGG181150.019.796785
AGTACCG318300.019.7577385
CCTAGAC373000.018.8982453
TCTTCTG3860300.018.254494-95
TAGAGCG354850.018.1242395
ACTGTCC214700.017.986198
TACCGTG287100.017.8678177
GTCTATG411200.017.4861091
TCTGCTT1810100.017.35506694-95
GTCTTCT4583400.017.29915894-95
GGACTGC280550.017.0321966
CTAGACA405400.016.9539414
TATACTG547450.016.3984685
CGTCTTC5486000.016.1466994-95
ACTGAGC478600.016.1272898
Produced by FastQC (version 0.11.5)