FastQCFastQC Report
Thu 12 Jul 2018
HVF5GBGX5_n01_nutrinetatrepmh-1_ln_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_nutrinetatrepmh-1_ln_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22482820
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGC844240.3755044963220806TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG109800.057.51541546
CGTATGC116800.054.10189444
GTATGCC120750.052.36016545
CTAATCG126300.050.0230133
ATGCCGT128150.049.5799647
TCGTATG128150.049.4739943
GCACACG129450.049.3631611
ACGTCTG131800.048.51204315
TGCCGTC135350.046.8645248
CACACGT138250.046.297212
ACACGTC139850.045.7679313
CTCGTAT141550.044.3693442
ACTAATC146800.043.2276832
GCCGTCT147700.043.08659749
CACTAAT150500.042.4435531
CACGTCT158750.040.42960414
CGTCTGA161600.039.84855316
AGCACAC163050.039.53420610
TCTCGTA160450.038.83675841
GTCACTA166750.038.2465329
Produced by FastQC (version 0.11.2)