FastQCFastQC Report
Thu 12 Jul 2018
HVF5GBGX5_n01_nutrinetatrepmh-1_ln_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_nutrinetatrepmh-1_ln_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12823339
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC552910.4311747509755454TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG67000.061.23768246
CGTATGC74750.054.66021744
GTATGCC76450.053.8556645
ATGCCGT76000.052.5114347
TCGTATG77900.051.5512743
TGCCGTC77500.050.5910648
ACGTCTG92200.049.0031615
GCACACG92500.048.91646611
GCCGTCT80600.048.1214349
CTCGTAT84850.047.49268342
CACACGT97450.046.360112
ACACGTC98150.046.0292813
AGCACAC111000.041.11051610
CACGTCT109950.041.02585614
CGTCTGA110800.040.68271616
TCGGAAG114250.039.9409073
TCTCGTA104050.039.03078541
GAGCACA118250.038.8859339
GATCGGA121350.037.2617571
ATCGGAA125000.036.3121532
Produced by FastQC (version 0.11.2)