FastQCFastQC Report
Thu 12 Jul 2018
HVF5GBGX5_n01_nutrinetatrepkn-0_ln_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_nutrinetatrepkn-0_ln_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11344032
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC308780.27219598816364415TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG38100.057.7008646
CGTATGC43700.050.2294744
GTATGCC44900.048.886645
ATGCCGT46200.047.6600347
TCGTATG49150.044.87345543
TGCCGTC53400.041.1024948
CTCGTAT54350.040.25753842
ACGTCTG57550.039.1624115
GCACACG59800.037.57037411
GCCGTCT58600.037.45318649
AACTAAT59650.037.14436735
ACTAATC60700.036.09977736
ACACGTC63750.035.40685313
CACACGT64200.035.21349712
CCAACTA63150.035.19499633
CACGTCT72050.031.62004714
CAACTAA72750.030.79072434
CGTCTGA74050.030.62613716
CTAATCT73600.030.20067837
TCTCGTA73050.030.04696741
Produced by FastQC (version 0.11.2)