FastQCFastQC Report
Thu 12 Jul 2018
HVF5GBGX5_n01_nutrinetatrepkn-0_ln_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_nutrinetatrepkn-0_ln_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13416503
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC1203410.8969624946232263TruSeq Adapter, Index 16 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG140400.064.2288246
CCCGTCC146450.062.40394233
CGTATGC147500.061.2852444
GTATGCC148200.060.87629345
CACCCGT152500.060.1089531
ACCCGTC153050.059.82612232
ATGCCGT151150.059.4062447
TCACCCG155500.058.9929930
TCGTATG152800.058.47192443
GTCACCC158400.058.0065129
TGCCGTC159300.056.36693648
CTCGTAT157300.055.37350542
ACGTCTG167650.055.3191315
GCCGTCT163800.054.23913249
CCGTCCA172700.052.8972434
GCACACG176150.052.80494711
CACACGT176800.052.47265212
ACACGTC179700.051.50913
CGTCTGA185550.049.88949216
TCTCGTA172750.049.5484241
Produced by FastQC (version 0.11.2)