FastQCFastQC Report
Thu 12 Jul 2018
HVF5GBGX5_n01_nutrinetatrepkn-0_ln_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_nutrinetatrepkn-0_ln_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12567061
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC393620.31321563570034394TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG49950.057.4679746
CGTATGC57350.050.23926544
GTATGCC58600.049.46533645
ATGCCGT57500.049.2522947
TCGTATG61200.046.73556543
ACGTCTG68100.044.50382215
TGCCGTC65700.043.5313848
GCACACG70400.043.19779211
CACACGT72800.041.62964212
GCCGTCT69950.040.73459649
CTCGTAT71100.040.12896742
ACACGTC77700.039.13915613
CACGTCT84850.035.71702614
CGTCTGA85750.035.54847316
AGCACAC89950.034.2760610
GAGCACA92450.033.9167189
TCTCGTA88350.032.57040441
TCGGAAG96450.031.5664883
CATCTCG95050.030.53186839
GATCGGA101200.029.880231
Produced by FastQC (version 0.11.2)