FastQCFastQC Report
Thu 12 Jul 2018
HVF5GBGX5_n01_nutrinetatrepge-0_ln_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_nutrinetatrepge-0_ln_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12652187
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGC298660.2360540513667716TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG37150.057.57614546
CGTATGC45600.046.7555644
GTATGCC46350.046.22504445
ATGCCGT49100.043.84823647
TCGTATG49050.043.53866243
TGCCGTC55400.038.8614248
ACGTCTG56550.038.43101515
CTCGTAT56500.037.9210542
GCACACG59900.036.45553611
GCCGTCT60400.035.7587849
CACACGT62600.034.8833112
ACACGTC63650.034.25274313
CACGTCT71900.030.3223714
CGTCTGA72500.030.07368516
TCTCGTA76850.028.01538741
AGCACAC81550.027.4638710
GAGCACA87450.025.890989
CAGTCAC93950.023.43468527
ATCTCGT92100.023.338140
GATCTCG92300.023.2494239
Produced by FastQC (version 0.11.2)