FastQCFastQC Report
Thu 12 Jul 2018
HVF5GBGX5_n01_nutrinetatrepge-0_ln_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_nutrinetatrepge-0_ln_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11977158
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT1570061.3108785907307894TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG168500.065.3799248
CGTATGC175550.063.11560446
ATGCCGT174150.062.63276349
GTATGCC177350.062.63136347
TCGTATG179750.061.44892545
TGCCGTC180200.060.1003250
ACGTCTG207750.059.68525315
CTCGTAT188800.059.15340844
GCACACG210800.058.8841611
GCCGTCT182150.057.84101551
CACACGT215150.057.79144712
ACACGTC216850.057.257713
GTCAGAA218900.056.25620736
CACGTCT221700.055.98979614
ATGTCAG219500.055.97040634
CGTCTGA222400.055.78582416
ACATGTC220700.055.77826332
GTCACAT226400.054.4528529
TGTCAGA227250.054.21859435
TCTCGTA206450.054.078843
Produced by FastQC (version 0.11.2)