FastQCFastQC Report
Thu 12 Jul 2018
HVF5GBGX5_n01_nutrinetatrepge-0_ln_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_nutrinetatrepge-0_ln_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13293133
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG18050.038.5942446
GTCACGC24950.028.61982529
CGTATGC25500.027.87051244
TCACGCC28000.025.3750130
GTATGCC28150.025.37033345
ATGCCGT28450.024.85492547
TCGTATG32100.022.576343
CACGCCA34500.021.10185431
TGCCGTC35000.020.40338348
TATCTCG37550.019.29914539
CTCGTAT40500.018.06676542
CGCCAAT40650.017.65094833
GCCGTCT40700.017.28716549
GCGGGCC3307.2759576E-1216.9691281
ACGCCAA43450.016.75514832
AGTCACG46850.015.68984828
ACGTCTG48400.015.25706415
GCGGGGT5850.014.9567631
GCCGGAT26450.013.232071
GCCAATA57300.012.82713334
Produced by FastQC (version 0.11.2)