FastQCFastQC Report
Thu 12 Jul 2018
HVF5GBGX5_n01_nutrinetatrepct-1_ln_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_nutrinetatrepct-1_ln_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11834853
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT330280.2790740197618002TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG43250.054.5545748
CGTATGC46700.050.52751546
GTATGCC48800.048.5663447
ATGCCGT52300.045.44681549
TCGTATG54600.043.34737845
ACGTCTG59850.042.2175215
TATCTCG58950.040.68134741
TGCCGTC59850.039.6532550
CTCGTAT61550.039.07945344
GCACACG67000.037.60537311
CACACGT67900.037.3650812
ACACGTC69050.036.64142213
GCCGTCT64800.036.6232651
CACACTG66850.036.2848231
CGTCTGA78400.032.40793216
CACGTCT79700.031.8770314
TGATATA76850.031.52002736
ACACTGA79850.030.59637532
ACTGATA80100.030.32570634
AGCACAC85200.029.77778810
Produced by FastQC (version 0.11.2)