FastQCFastQC Report
Thu 12 Jul 2018
HVF5GBGX5_n01_nutrinetatrepct-1_ln_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_nutrinetatrepct-1_ln_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15869247
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT2987491.8825656945159401TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG323800.066.3387848
CGTATGC331300.064.8396646
ATGCCGT334350.064.1784849
GTATGCC335200.064.1360747
TCGTATG338650.063.23737345
TATCTCG341400.062.53117841
TCCGTAT345350.062.34713737
TGCCGTC344900.062.11150450
CGTATCT345000.061.8964439
CTCGTAT345950.061.83350444
ACGTCTG358150.061.64723615
CCGTATC350950.061.20479238
GTTCCGT353850.060.98412335
GCACACG364050.060.76082611
GCCGTCT351150.060.6465751
CACACGT366950.060.25221312
TTCCGTA358300.060.2389536
AGTTCCG362350.059.64752634
ACACGTC371000.059.6033413
CGTCTGA376950.058.58377516
Produced by FastQC (version 0.11.2)