FastQCFastQC Report
Thu 12 Jul 2018
HVF5GBGX5_n01_nutrinetatrepct-1_ln_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_nutrinetatrepct-1_ln_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13403967
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1070350.7985322554136398TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG135300.063.88244246
CGTATGC141700.061.02526544
ATGCCGT143650.059.85075847
GTATGCC145200.059.72215745
TCGTATG147000.058.3965743
TGCCGTC150200.056.9837448
CTCGTAT140050.055.8191642
GCCGTCT156300.054.5995749
ACGTCTG164250.054.5892615
GCACACG173000.052.08831411
CACACGT174350.051.6249712
CACAGTG171000.051.58099733
ACACGTC175750.051.17371713
ACACAGT174500.050.46618732
CACACAG175850.050.27686731
CGTCTGA180650.049.7701916
CACGTCT180750.049.7000414
TCTCGTA165000.047.41964341
AGCACAC194950.046.4030410
TCACACA191450.046.28878830
Produced by FastQC (version 0.11.2)