FastQCFastQC Report
Thu 12 Jul 2018
HVF5GBGX5_n01_nutrinetatrepcol-0_ln_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_nutrinetatrepcol-0_ln_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12280203
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC210370.17130824303148734TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG29350.049.61761546
CGTATGC34850.041.8901844
GTATGCC37200.039.2430945
ATGCCGT38700.037.53867747
TCGTATG40950.035.6501343
TGCCGTC41250.035.38725348
GCACACG47100.032.2461611
ACGTCTG47050.031.9103515
CTCGTAT45850.031.61022642
GCCGTCT47800.030.97667949
ACGGTAG50700.028.99558432
CACACGT53300.028.75794412
AGTCACG52350.028.34970528
ACACGTC54450.028.2146113
GTCACGG53350.027.88392429
GGTAGCA56450.026.28936634
CACGTCT63200.023.9760814
CGTCTGA63800.023.58835216
AGCACAC68450.023.05750310
CGGTAGC64500.022.90050533
Produced by FastQC (version 0.11.2)