FastQCFastQC Report
Thu 12 Jul 2018
HVF5GBGX5_n01_nutrinetatrepcol-0_ln_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_nutrinetatrepcol-0_ln_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11781102
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1325511.1251154603363929TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG147750.065.5107548
CGTATGC154350.062.78021246
GTATGCC154500.062.71740347
ATGCCGT157300.061.64185349
TCGTATG160500.060.4197845
TGCCGTC163250.059.41510850
CTCGTAT164350.058.77184744
GCCGTCT170450.056.82200251
ACGTCTG176500.055.51929515
CACACGT182100.054.05811712
GCACACG182200.054.0474311
TCTCGTA180100.053.55463443
ACACGTC185150.053.09155713
CACAGTC183800.052.8460631
ACAGTCA187250.052.04107332
CACGTCT191150.051.4804314
CGTCTGA191800.051.2014916
AATCTCG192350.050.19621741
GTCACAG195800.049.78837229
ATCTCGT194400.049.6143342
Produced by FastQC (version 0.11.2)