FastQCFastQC Report
Thu 12 Jul 2018
HVF5GBGX5_n01_nutrinetatrepcol-0_ln_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_nutrinetatrepcol-0_ln_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13911735
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC573110.4119615561969805TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG68100.060.3495846
CGTATGC74650.055.1520944
GTATGCC78100.052.8935845
ATGCCGT77950.052.6779547
TCGTATG83050.049.53146443
TGCCGTC84200.048.9340748
GCCGTCT89250.046.08498849
CTCGTAT90850.044.85291342
ACTGACC93450.044.12232
CACTGAC94500.043.74287431
ACGTCTG97050.042.912115
AGTCACT106450.039.1957128
ACACGTC106700.039.02928513
CACACGT107600.038.800412
GCACACG108050.038.67119611
TCTCGTA107700.037.8669541
CGTCTGA114850.036.2622916
GTCACTG115650.036.0771629
CACGTCT115900.036.05222314
CCGTCTT124700.033.23493250
Produced by FastQC (version 0.11.2)