FastQCFastQC Report
Thu 12 Jul 2018
HVF5GBGX5_n01_nutrinetatrepbur-0_ln_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_nutrinetatrepbur-0_ln_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12610378
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT225310.1786702983843942TruSeq Adapter, Index 22 (97% over 40bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145010.1149925878510541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG32200.050.33418748
CGTACGT34050.047.5947234
CCGTACG36650.044.31466733
GTATGCC37100.044.15839447
GTACGTA37350.043.39076635
CGTATGC38850.041.80935746
ATGCCGT42600.038.62016349
TACGTAA43900.036.9176236
TCGTATG44250.036.70821845
ACGTCTG47950.035.033815
TGCCGTC50550.032.8221950
ACCGTAC50450.032.4703132
CTCGTAT51300.031.66364544
GCACACG54100.031.05034811
GCCGTCT52900.031.03265451
ACACGTC56850.029.60932713
CACACGT60300.027.91581312
ACGTAAT59250.027.53076437
CACGTCT66800.025.25134514
CGTCTGA68500.024.8816916
Produced by FastQC (version 0.11.2)